 ผลงานตีพิมพ์ในวารสารวิชาการDevelopment and application of an indirect enzyme-linked immunosorbent assay for serological survey of Japanese encephalitis virus infection in dogsผู้แต่ง: Hiroshi Shimoda,  Ms.natnaree inthong, Assistant Professor , Keita Noguchi, Yutaka Terada, Yumiko Nagao, Masayuki Shimojima, Tomohiko Takasaki, Worawut Rerkamnuaychoke, Ken Maedaa, วารสาร:   
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ผลงานตีพิมพ์ในวารสารวิชาการDevelopment and evaluation of indirect enzyme-linked immunosorbent assay using recombinant dense granule antigen 7 protein for the detection of Toxoplasma gondii infection in cats in Thailandผู้แต่ง:Dr.Eukote Suwan, Assistant Professor , Chalermwong, P., Dr.Rucksak Rucksaken, Assistant Professor, Dr.Metita Sussadee, Assistant Professor, Dr.Sarawan kaewmongkol, Associate Professor , Udonsom, R., Dr.Sathaporn Jittapalapong, Professor , Mr.Bandid Mangkit, Assistant Professor , วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Indirect Enzyme-Linked Immunosorbent Assay Test Kit Development for Specific Antibody Detection Against Brucella abortus in Cattle) ผู้เขียน:Monaya Ekgatat, Suree Thammasart, Reka Kanitpun, Surapong Wongkasemjit, Chokchai Nokdhes, Utit Trenuntawan สื่อสิ่งพิมพ์:pdf AbstractThe smooth lipopolysaccharide (SLPS) antigen, prepared from Brucella abortus strain 99 was used in an indirect Enzyme-Linked Immunosorbent Assay (iELISA). The data were expressed as optical density and generated using computer software. The positive results were accepted at the cut-off values of ? 40 percent positivity (PP). The strong positive serum control (C++), weak positive serum control (C+), negative serum control (C-), and conjugate control (Cc) were used to optimize the test kit. The iELISA was optimization and standardization for antigen concentration, test material dilution and detection system dilution by checkerboard titration. The statistic evaluation using data from sera from 317 infected and 5,300 non-infected cattle revealed that the sensitivity (Se) is 99.369 (98.497-100.0)%, specificity (Sp) is 99.887 (99.796-99.977)%, accuracy (Ac) is 99.858% and Kappa value is 0.987. The iELISA test kit was subsequently validated by testing 9,877 field serum samples submitted to laboratory. The complement fixation test was used as gold standard. The results revealed that the relative Se is 99.026 (97.929-100.0)%, relative Sp is 98.547 (98.308-98.787)%, relative Ac is 98.562% and Kappa value is 0.804 when cattle sera were tested. The components of this iELISA test kit consisted of antigen, control sera, and essential diluents for 800 tests. This test showed high sensitivity, specificity, and accuracy and could be performed in mass. Therefore, this iELISA test kit might be an appropriate tool for brucellosis control in Thailand. |
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Researcherนาย บัณฑิต มังกิจ, ผู้ช่วยศาสตราจารย์ที่ทำงาน:ภาควิชาเทคนิคการสัตวแพทย์ คณะเทคนิคการสัตวแพทย์ สาขาที่สนใจ:Veterinary and Medical Parasitology (Helminths, Ectoparasites, and Protozoa), Molecular parasitology Resume |
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